EFHSS Questions and Answers - Question Q00339

Dear Sir,

Would you please inform In Plasma sterilization what is the role of Hydrogen Peroxide (50%) sterilant in the Sterilization process. And what is the role of Plasma Generated by RF. What is it that is responsible to kill the Microorganism? Is it the Plasma or the Hydrogen Peroxide Sterilant? Would you please explain to me the role of Plasma and Hydrogen Peroxide in this case seperately.

Thanks and best regards,
Mr. Agnelo
Kuwait

Mr. Angelo,

Hydrogen Peroxide Gas Plasma Sterilization is based on a process involving the generation of law temperature gas plasma from hydrogen peroxide. It is a complicated process.
After a vacuum is drawn sterilization begins with the injections of a solution of 58% (normal) hydrogen peroxide into the chamber.
Hydrogen peroxide vaporizes in the vacuum and diffuses throughout the chamber, surrounding items to be sterilized and initiating inactivation of microorganisms in the sterilizer.
Radio-frequency (RF) energy is then applied to create an electric field, which in turn transforms the hydrogen peroxide into a low-temperature gas plasma state.
In this gas plasma physical state, hydrogen peroxide vapour breaks apart into reactive species known as free radicals, which interact with the cell membranes, enzymes, or nucleic acids to disrupt life functions of microorganisms. The plasma is maintained for sufficient time to achieve sterilization.
After the activated components react with microorganisms, they lose their high energy, and recombine to form oxygen, water and other non-toxic by- products.
"Mini" Explanation: The plasma arc "activates" the hydrogen peroxide, enhancing the efficacy.

Hope this is of some help!
Josy Holdener